Automated fluorescent scanning & analysis of target cells in suspension, previously stained with a different marker
BioView’s Target FISH (T-FISH) application provides a genetic content analysis (FISH) of a specific, pre-selected subset of target cells. First the application scans slides stained with Giemsa morphology stain, Immuno fluorescent antibodies (IFL) or FISH probes, for the detection and selection of specific cell types/groups, then it rescans the same slides after they have been de-stained and stained again with different Immuno fluorescent antibodies or FISH probes. The two scans are matched so to present two images of the same cell for each and every cell in the sample (morphology/FISH or IFL/FISH or FISH/FISH).
The T-FISH method is applied to the diagnosis and follow-up of:
– MRD in Leukemia patients
– Multiple Myeloma
– Acute Myeloid Leukemia
– Bone Marrow transplantation
– Sex-mismatched and specific chromosomal aberration
– Severe Combined Immunodeficiency (SCID) syndrome
– Autologus stem cells transplantation
The T-FISH method is also utilized in the following cases:
– FISH analysis of samples consecutively / simultaneously stained with immuno fluorescent antibodies and FISH markers.
– FISH analysis of cells consecutively hybridized with a mixture of several different FISH probes, to provide multiple FISH analysis of the same cells (multiplexing)